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Why I Still Use Python Virtual Environments in Docker (via) Hynek Schlawack argues for using virtual environments even when running Python applications in a Docker container. This argument was most convincing to me:

I'm responsible for dozens of services, so I appreciate the consistency of knowing that everything I'm deploying is in /app, and if it's a Python application, I know it's a virtual environment, and if I run /app/bin/python, I get the virtual environment's Python with my application ready to be imported and run.

Also:

It’s good to use the same tools and primitives in development and in production.

Also worth a look: Hynek's guide to Production-ready Docker Containers with uv, an actively maintained guide that aims to reflect ongoing changes made to uv itself.

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